Batman and Robin fail to stop Mr. Freeze from stealing a cache of diamonds. They learn that Freeze was once a scientist named Victor Fries, who became dependent on a diamond-powered subzero suit following an accident in a cryogenics lab while working to save his wife, Nora, from a terminal illness called MacGregor’s Syndrome. Meanwhile, botanist Dr. Pamela Isley is experimenting with the strength serum “Venom” to create mutant plants capable of fighting back against mankind. She is angry that her senior colleague Dr. Jason Woodrue used her Venom to transform a diminutive prisoner into the “super soldier” Bane. She refuses to partner with Woodrue so he tries to kill her with animal-plant toxins and chemicals, causing her to transform into the beautiful Poison Ivy. She kills Woodrue with a venomous kiss and vows to establish botanical supremacy over the world. Alfred Pennyworth’s niece Barbara Wilson makes a surprise visit from England and is invited to stay at Wayne Manor. Later, Barbara finds the Batcave and creates her own crime-fighting persona with the help of a computer simulation of Alfred. The real Alfred is suffering from MacGregor’s Syndrome. He is, however, in stage 1, for which Mr. Freeze has developed a cure despite being unable to cure his wife’s condition because it is too advanced. Ivy arrives in Gotham City with Bane as her henchman. She interrupts a Wayne Enterprises press conference at the Gotham Observatory where a giant telescope is being unveiled. Ivy demands Bruce Wayne use his fortune to safeguard the natural environment at the expense of millions of human lives, and Bruce refuses. Ivy appears at the Gotham Botanical Gardens fundraiser, seducing everyone present with her pheromone dust, including the Dynamic Duo, who are there to protect a diamond from Mr. Freeze. When Freeze crashes the event Ivy is instantly captivated by his “ruthless charm”. Freeze is captured by Batman and detained at the Arkham Asylum but is released by Ivy. Ivy turns off Nora Fries’ life support and makes Freeze believe Batman did it, persuading him that they should destroy Batman along with the society that created him. They plan to turn the observatory’s new telescope into a giant freeze ray to kill all humanity to allow Ivy’s mutant plants to take over the world. Meanwhile, Robin is under Ivy’s seductive spell and is rebelling against Batman. Robin goes to meet Ivy at her garden hideout, where her venomous kiss fails to kill Robin because Batman had prevailed on him to coat his lips with rubber. Ivy tries to drown Robin in her lily pond and entangles Batman in her crushing vines, but they are able to free themselves when Batgirl arrives and traps Ivy in her own floral throne. Batgirl reveals herself as Barbara. The three crime-fighters arrive at the Observatory to stop Freeze who has already frozen all of Gotham. Bane attacks Robin and Batgirl, but they incapacitate him and restore him to his original human state. Robin and Batgirl save Gotham by using the observatory’s satellites to reflect sunlight from outer space to thaw the city. Batman shows Freeze video proof that Ivy pulled the plug on Nora and reveals that Batman was the one who saved her. He vows that Freeze will be allowed to continue his research at Arkham Asylum to cure Nora. Batman asks Freeze for his cure for the first stage of MacGregor’s Syndrome for Alfred and Freeze atones for his misdeeds by giving him two vials of the medicine. At Arkham, Ivy is joined in her cell by Freeze, who vows to exact revenge on her. Back at Wayne Manor, Alfred is cured and Bruce invites Barbara to live with them, joining Batman and Robin to fight crime as Batgirl.
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